|
KMID : 0880220230610040379
|
|
Journal of Microbiology
2023 Volume.61 No. 4 p.379 ~ p.388
|
|
|
Descr!ption of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β?cryptoxanthin Isolated from the Tongtian River Sediments
|
|
Choi Seung-W. Scott
Yifan Jiao
Sihui Zhang
Yuanmeihui Tao
Suping Zhang
Dong Jin
Ji Pu
Liyun Liu
Lee Boung-Chul
Shan Lu
|
|
|
Abstract
|
|
|
|
Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37′13″N, 96°05′37″E) in July 2019. Both strains were shown to grow at 15?35 °C and pH 7.0?10.0, and in the presence of 0?6.0% (w/v) NaCl. The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6?98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5?98.6%) and O. murale 01-Gi-040T (98.3?98.5%). The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0?23.9% and 70.8?80.4%, respectively, all below the respective recommended 70.0% and 95?96% cutoff point. Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9. Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids. β-cryptoxanthin ( C40H56O) can be extracted from strain JY.X270T, and its content is 6.3 μg/ml. Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp. nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).
|
|
|
KEYWORD
|
|
|
Ornithinimicrobium cryptoxanthini, Sediment, β-cryptoxanthin, Genomic characteristics, Taxonomy
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|